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KMID : 0620920080400060583
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Experimental & Molecular Medicine
2008 Volume.40 No. 6 p.583 ~ p.595
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Polarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus
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Han Young-Woo
Aleyas Abi G.
George Junu A.
Yoon Hyun-A
Eo Seong-Kug
Yoo Dong-Jin
Kim Koan-Hoi
Kim Seon-Ju
Kim Hye-Kyung
Kang Seong-Ho
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Abstract
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Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protective immunity against a lethal challenge. Among three constructs, recombinant adenovirus expressing gB (rAd-gB) was found to induce the most potent immunity biased to Th1-type, as determined by the IgG isotype ratio and the profile of the Th1/Th2 cytokine production. Conversely, the gC-expressing adenovirus (rAd-gC) revealed Th2-type immunity and the gD-expressing adenovirus (rAd-gD) induced lower levels of IFN-? and IL-4 production than other constructs, except IL-2 production. Mucosal delivery of rAd-gB induced mucosal IgA and serum IgG responses and biased toward Th2-type immune responses. However, these effects were not observed in response to systemic delivery of rAd-gB. In addition, rAd-gB appeared to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route. These results suggest that administration of replication-incompetent adenoviruses can induce different types of immunity depending on the expressed antigen and that recombinant adenoviruses expressing gB induced the most potent Th1-biased humoral and cellular immunity and provided effective protection against PrV infection.
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KEYWORD
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adenoviruses, human, herpesvirus 1, Suid, Th1 cells, Th2 cells, vaccination
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